Genetic Polymorphism of The Gnrh Gene and Its Relationship With Productive Traits and Performance of Japanese Quail
DOI:
https://doi.org/10.51699/ijbea.v4i3.661Keywords:
Japanese quail, GnRH gene, genetic polymorphism, growth traits, feed conversion efficiency.Keywords: Quail bird, GnRH gene, Quail WeightAbstract
This study investigates the genetic polymorphism of the gonadotropin-releasing hormone (GnRH) gene and its association with growth and performance traits in Japanese quail. The research involved both field and laboratory phases, including the weekly monitoring of 200 birds for body weight, length, and feed conversion efficiency. Following the rearing phase, blood samples were collected for molecular analysis. DNA was extracted and a 906 bp segment of the GnRH gene was amplified using PCR. Electrophoresis confirmed successful amplification. Sequence analysis revealed three single nucleotide polymorphisms (SNPs): 67A>G, 277G>C, and 323C>T. The study found statistically significant associations between certain genotypes and specific performance traits. Notably, the GG genotype of 67A>G was significantly associated with higher body weight during the first and third weeks, while the CC genotype of 323C>T showed superior weight performance in the second week. In contrast, feed conversion efficiency was significantly improved in the TT genotype of 323C>T during the fourth and fifth weeks, and the GG genotype of 67A>G during the fifth week. No significant associations were observed for bird length across all genotypes and weeks. These findings underscore the potential of using genetic markers in selective breeding programs to enhance the productivity of Japanese quail.
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